ctni mca1208 Search Results


ctni  (Bio-Rad)
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Data show the mean ± SD with each sample as dot plots for the protein abundance of <t>phospholamban</t> <t>(PLN,</t> B), phospho-PLN-T17 (C), ratio of phospho-PLN-T17 to PLN (D), cardiac troponin I <t>(cTnI,</t> E), phospho-cTnI (F), ratio of phospho-cTnI to cTnI (G), calsequestrin-1 (H), SERCA2 (I), ratio of SERCA2 to PLN (J), sodium-calcium exchanger 1 (NCX1, K), L-type calcium channel (LTCC, L), ryanodine receptor 2 (RYR2, M), phospho-RYR2-S2808 (N), ratio of phospho-RYR2-S2808 to RYR2 (O) and Connexin43 (Cx43, P). Data was analysed using an unpaired Student’s T-Test for all proteins except PLN, cTnI, NCX and phospho-cTnI/cTnI ratio which used a Mann-Whitney test (data not normally distributed). P<0.05 was considered significant. Normoxia = closed circles, Hypoxia = open circles.
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Data show the mean ± SD with each sample as dot plots for the protein abundance of phospholamban (PLN, B), phospho-PLN-T17 (C), ratio of phospho-PLN-T17 to PLN (D), cardiac troponin I (cTnI, E), phospho-cTnI (F), ratio of phospho-cTnI to cTnI (G), calsequestrin-1 (H), SERCA2 (I), ratio of SERCA2 to PLN (J), sodium-calcium exchanger 1 (NCX1, K), L-type calcium channel (LTCC, L), ryanodine receptor 2 (RYR2, M), phospho-RYR2-S2808 (N), ratio of phospho-RYR2-S2808 to RYR2 (O) and Connexin43 (Cx43, P). Data was analysed using an unpaired Student’s T-Test for all proteins except PLN, cTnI, NCX and phospho-cTnI/cTnI ratio which used a Mann-Whitney test (data not normally distributed). P<0.05 was considered significant. Normoxia = closed circles, Hypoxia = open circles.

Journal: bioRxiv

Article Title: Developmental Hypoxia Increases Susceptibility to Cardiac Ventricular Arrhythmias in Adult Offspring

doi: 10.64898/2026.01.22.701057

Figure Lengend Snippet: Data show the mean ± SD with each sample as dot plots for the protein abundance of phospholamban (PLN, B), phospho-PLN-T17 (C), ratio of phospho-PLN-T17 to PLN (D), cardiac troponin I (cTnI, E), phospho-cTnI (F), ratio of phospho-cTnI to cTnI (G), calsequestrin-1 (H), SERCA2 (I), ratio of SERCA2 to PLN (J), sodium-calcium exchanger 1 (NCX1, K), L-type calcium channel (LTCC, L), ryanodine receptor 2 (RYR2, M), phospho-RYR2-S2808 (N), ratio of phospho-RYR2-S2808 to RYR2 (O) and Connexin43 (Cx43, P). Data was analysed using an unpaired Student’s T-Test for all proteins except PLN, cTnI, NCX and phospho-cTnI/cTnI ratio which used a Mann-Whitney test (data not normally distributed). P<0.05 was considered significant. Normoxia = closed circles, Hypoxia = open circles.

Article Snippet: Membranes were incubated with primary antibodies (PLN [1/5000 dilution] – MA3-922, InVitrogen; phospho-PLN-T17 [1/1000 dilution] – AP0910, ABclonal; cTnI [1/1000 dilution] – MCA1208, Bio-Rad; phospho-cTnI [1/1000 dilution] – MCA2780, Bio-Rad; Calsequestrin-1 [1/5000 dilution] – PA1-913, InVitrogen; SERCA2 [1/2000 dilution] – ab3625, Abcam; NCX1 [1/1000 dilution] – R3F1, Swant; LTCC [1/1000 dilution] – ab2864, Abcam; RYR2 [1/1000 dilution] – ab302716, Abcam; phospho-RYR2-S2808 [1/1000 dilution] – PA5-105712, InVitrogen) overnight at 4°C, before secondary antibody incubation (IRDye® 800CW Goat anti-mouse for PLN, cTnI, phospho-cTnI, NCX1 and LTCC, IRDye® 800CW goat anti-rabbit for phospho-PLN-T17, Calsequestrin-1, SERCA2, RYR2 and phosphor-RYR2-S2808, both 1/20 000 dilution) at room temperature for one hour.

Techniques: Quantitative Proteomics, MANN-WHITNEY